Professor of Molecular Science

 

 

Publications since 2001

•  Van Trappen, PO, Gyselman, VG, Lowe, DG, Ryan, A, Oram, DH, Bosze, P, Weekes, AR, Shepherd, JH, Dorudi, S, Bustin, SA, Jacobs, I.J. ( 2001 ) Molecular quantification and mapping of lymph-node micrometastases in cervical cancer Lancet 357: 15-20

•  Korbonits, M, Bustin , SA , Kojima, M, Jordan , S, Adams, EF, Lowe, DG, Kangawa, K, Grossman AB ( 2001 ) The Expression of the Growth Hormone Secretagogue Receptor Ligand Ghrelin in Normal and Abnormal Human Pituitary and Other Neuroendocrine Tumors. J Clin Endocrinol and Metabolism 86:881-887

•  Bustin, SA, Jenkins, PJ ( 2001 ) The growth hormone/insulin-like growth factor-I axis and colorectal cancer. Trends Mol Med 7 447-454

•  Miraki-Moud F, Jenkins PJ, Fairclough PD, Jordan S, Bustin SA, Jones AM, Lowe DG, Monson JP, Grossman AB , Besser GM, Camacho-Hubner C. ( 2001 ) Increased levels of insulin-like growth factor binding protein-2 in sera and tumours from patients with colonic neoplasia with and without acromegaly. Clin Endocrinol 54:499-508

•  Bustin, SA, Li SR, Phillips, S, Dorudi S ( 2001 ) Expression of HLA class II in colorectal cancer: evidence for enhanced immunogenicity of MSI + tumours. Tumor Biol. 22:294-298

•  Bustin, SA, Li SR, Dorudi S ( 2001 ) Expression of the Ca 2+ -activated Chloride Channel genes CLCA1 and CLCA2 is down-regulated in human colorectal cancer. DNA Cell Biol 20 331-338

•  Ogunkolade BW, Boucher BJ, Prahl JM, Bustin SA , Burrin JM, Noonan K, North BV, Mannan N, McDermott MF, DeLuca HF, Hitman GA ( 2002 ) Vitamin D receptor mRNA and VDR protein levels in relation to vitamin D status, insulin secretory capacity and VDR genotype in Bangladeshi Asians. Diabetes 51:2294-2300

•  Tricarico, C, Pinzani, P, Bianchi, S, Paglierani, M, Distante, V, Pazzagli, M, Bustin, SA , Orlando , C. ( 2002 ) Quantitative real-time RT-PCR: Normalisation to rRNA or single housekeeping genes is inappropriate for human tissue biopsies. Anal Biochem . 309:293-300

•  Kirkpatrick KL, Ogunkolade W, Elkak A, Bustin SA , Jenkins P, Ghilchik M, Mokbel K. ( 2002 ) The mRNA expression of cyclo-oxygenase-2 (COX-2) and vascular endothelial growth factor (VEGF) in human breast cancer. Curr Med Res Opin 18:237-241

•  Bustin, SA ( 2002 ) Quantification of mRNA using real-time RT-PCR: trends and problems. J Mol Endocrinol 29:23-39

•  Bustin, SA and Dorudi, S ( 2002 ) The value of quantitative gene profiling data for molecular diagnostics. Trends Mol Med 8:269-272

•  Bustin, SA ( 2002 ) Nucleic Acid-Based Technologies: time to stop and think. Exp Rev Mol Diagn . 2:89-92

•  Bustin, SA, Dorudi S, Phillips, S, Feakins, R, Jenkins, PJ ( 2002 ) Local effects of insulin-like growth factor-I in normal colon and in colorectal cancer. Tumor Biol. 23:130-138

•  Gnanapavan, S, Kola, B, Bustin, SA , Morris, DG, McGee, P, Fairclough, P, Carpenter, R, Grossman AB, Korbonits M ( 2002 ) The tissue distribution of the mRNA of ghrelin and subtypes of its receptor, GHS-R, in humans J Clin Endocrinol and Metabolism 87:2988-2991

•  Ogunkolade, BW, Boucher, BJ, Fairclough, PD, Hitman, HA, Durudi, S, Jenkins, PJ, Bustin, SA ( 2002 ) Down-regulation of 25-hydroxyvitamin D-1-a-hydroxylase in normal colonic mucosa of Patients with colorectal cancer. Lancet 359:1831-1832.

•  Kirkpatrick KL, Ogunkolade W, Elkak AE, Bustin SA , Jenkins P, Ghilchik M, Newbold, RF, Mokbel K. ( 2003 ) hTERT expression in human breast cancer and non-cancerous breast tissue: correlation with tumour stage and c-Myc expression. Breast Canc Res and Treat 77:277-284

•  Li, S.-R, Dorudi, S, Bustin SA. ( 2003 ) Identification of differentially expressed genes associated with colorectal cancer liver metastases. Eur Surgical Res 35: 327-36

•  Laban C, Bustin SA , Jenkins PJ ( 2003 ) The GH/IGF-I axis in breast cancer. Trends Endocrinol Metab 14:28-34

•  Kola B, Korbonits M, Kaltsas G, Morris DG, Metherell L, Powell P, Czirják S, Arnaldi G, Bustin SA , Boscaro M, Mantero F, Grossman AB ( 2003 ) Growth Hormone Receptor And Type 1 Insulin-Like Growth Factor Receptor Expression In Human Somatotroph Tumors. Clin Endocrinol (Oxf). 59: 328-38

•  Vithana EN, Abu-Safieh L, Bird AC,. Hunt DM, Bustin SA , Bhattacharya SS ( 2003 ) Expression of precursor RNA processing factor 31 ( PRPF31 ) mRNA in patients with autosomal dominant retinitis pigmentosa: a molecular 'clue' for incomplete penetrance . Invest Ophthalmol Vis Sci. 44: 4204-4209

•  Banerjea A, Phillips SM, Dorudi S, Bustin SA . (2003) Colorectal cancers with mononucleotide microsatellite instability can be identified using microfabricated chip technology. Anal Biochem. 322: 130-133

•  Bustin SA , Siddiqi S, Ahmed S, Hands RE, Dorudi S (2004) Quantification of cytokeratin 20, carcinoembryonic antigen and guanylyl cyclase C mRNA levels in lymph nodes may not predict treatment failure in colorectal cancer patients. Int J Cancer 108:412-417

•  Phillips SM, Banerjea A, Feakins E, Li, S-R, Bustin SA , Dorudi S ( 2004 ) Antigen-specific activation of tumour infiltrating lymphocytes in colorectal cancer with microsatellite instability. Br J Surgery 91:469-475

•  Stamm DS, S. Dziennis S, Bustin SA , Sanderson IR (2004) Diet induces MHC class II expression in mouse intestinal epithelium through the regulatory molecule, CIITA IV Gastroenterology 127:203-212

•  Li, SR., Ng, CFJ, Banerjea, A, Ahmed, S, Hands, R, Powar, M, Ogunkolade, W. Dorudi, S, Bustin SA (2004) Differential expression patterns of the insulin-like growth factor II gene in human colorectal cancer Tumor Biology 25:62-68

•  Dheda, K., Huggett J, Bustin SA , Johnson M, Rook G, Zumla A ( 2004 ) Validation of housekeeping genes for normalising RNA expression in Tuberculosis. Biotechniques 37:112-119

•  Banerjea A, Ahmed S, Hands RE, Bustin SA, Dorudi S (2004) Colorectal Cancers with Microsatellite instability display mRNA expression signatures characteristic of increased immunogenicity. Mol Cancer 6:21-29

•  Jenkins, PJ and Bustin SA (2004) Evidence for a link between IGF-I and cancer Eur J Endocrinol 151:S17-22

•  Bustin, SA ( 2004 ) Reacting in real-time. The Endocrinologist 71:9

•  Bustin, SA and Nolan T (2004) Pitfalls of Quantitative Real-Time PCR. J Biomol 15:155-166

•  Bustin SA, Dorudi S ( 2004 ) Gene expression profiling for molecular staging and prognosis prediction in colorectal cancer. Exp Rev Mol 4:599-607

•  Banerjea A, Bustin SA , Dorudi S. ( 2005 ) The immunogenicity of colorectal cancers with high-degree microsatellite instability. World J Surg Oncol. 2005 May 12:26

•  Huggett J, Dheda K, Bustin SA , Zumla A. ( 2005 ) Real-time RT-PCR normalisation; strategies and considerations. Genes Immun. 6:279-84.

•  Bustin SA , Nolan T, Benes V, Pfaffl M ( 2005 ) Real-time fluorescence-based PCR, J Mol Endocrinol 34:597-601

  •  Bustin SA , Mueller R ( 2005 ) Real-time reverse transcription PCR and its potential use in clinical diagnosis. Clinical Science 109:365-379

•  Dheda K., Huggett J, Chang JS, Kim LU, Bustin SA , Johnson M, Rook G, Zumla A ( 2005 ) The implications of using an inappropriate reference gene for real-time RT-PCR data normalisation. Anal Biochem 344:141-143

•  Ahmed S, Banerjea A, Hands RE, Bustin SA , Dorudi ( 2005 ) Microarray profiling of colorectal cancer in Bangladeshi patients. Colorectal Disease 7:571-575

•  Bustin, SA ( 2005 ) Real-time fluorescence-based quantitative PCR: a snapshot of current procedures and preferences. Exp. Rev. Mol. Diagn. 5:493-498

•  Jenkins, PJ, Khalaf, S, Ogunkolade, W, McCarthy, K, David, T, Hands, RE, Davies, D, Bustin, SA (2005) Differential expression of IGFBP-3 in normal and malignant colon and its influence on apoptosis. Endocrine-related Cancer 12:1-11

•  Banerjea A, Feakins RM, Nickols CD, Phillips SM, Powar MP, Bustin SA, Dorudi S ( 2005 ) Immunogenic HSP-70 is overexpressed in colorectal cancers with high-degree microsatellite instability. Dis. Colon Rectum 48:2322-2328

•  Bustin, SA Mueller, R. (2006) Real-time reverse transcription PCR and the detection of occult disease in colorectal cancer. Mol. Aspects Medicine 27:192-223

•  Nolan T, Hands RE, Ogunkolade W, Bustin SA . (2006) SPUD: A quantitative PCR assay for the detection of inhibitors in nucleic acid preparations. Anal Biochem. 351:308-310.

•  Ogunkolade WB, Boucher BJ, Bustin SA, Burrin JM, Noonan K, Mannan N, Hitman GA. Vitamin D (2006) Metabolism in Peripheral Blood Mononuclear Cells is influenced by Chewing 'Betel nut' (Areca catechu) and Vitamin D Status. J Clin Endocrinol Metab. 91:2612-2617

•  McCarthy K, Bustin SA, Ogunkolade B, Khalaf S, Laban CA, McVittie CJ, Carpenter R, Jenkins PJ. (2006) Cyclo-oxygenase-2 (COX-2) mRNA expression and hormone receptor status in breast cancer. Eur J Surg Oncol . 32:707-709  

•  Bustin, SA. (2006) Nucleic acid quantification and disease outcome prediction in colorectal cancer. Personalized Medicine (2006) 3(2), 207-216

•  McVittie C, Khalaf S, Ogunkolade W, McCarthy K, Laban C, Macaulay VM, Carpenter R, Bustin SA, Jenkins P. Extended silencing of the insulin-like growth factor-I receptor gene in MCF-7 cells by serial transfection with siRNA. Anal Biochem. 2006 Oct 15;357(2):305-7

•  Nolan T, Hands RE, Bustin SA.  Quantification of mRNA using real-time RT-PCR. Nat Protoc. 2006;1(3):1559-82.

•  Nolan T, Hands RE, Ogunkolade W, Bustin SA.  SPUD: a quantitative PCR assay for the detection of inhibitors in 
nucleic acid preparations. Anal Biochem. 2006 Apr 15;351(2):308-10.

•  Bustin SA, Mueller R. Real-time reverse transcription PCR and the detection of occult  disease in colorectal cancer. Mol Aspects Med. 2006 Apr-Jun;27(2-3):192-223

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