Nitric Oxide Measurements

DAF-FM Loading Protocol

1. Pellet cells and adjust to million cells per ml.

2. Dissolve DAF-FM in DMSO (Invitrogen, Cat. No.D23841).

3. Adjust DAF-FM to a final concentration of 2 uM.

4. Incubate at 37C for 30 mins.

5. Wash cells in PBS.

6. Add PI (5 ug/ml)