Mitochondrial Membrane Potential Measurements

DiOC(3)6 Loading Protocol

 

1.   Pellet cells and adjust to million cells per ml.

2.   Dissolve DioC(6)3 in DMSO (Invitrogen, Cat. No.D273).

3.   Adjust DiOC(6)3 to a final concentration of 4 nM.

4.  Incubate at 37C for 30 mins.

5. Wash cells in PBS.

6. Add DAPI (200 ng/ml)