Mitochondrial Membrane Potential Measurements
DiOC(3)6 Loading Protocol
1. Pellet cells and adjust to million cells per ml.
2.
Dissolve DioC(6)3 in DMSO (Invitrogen, Cat. No.D273).
3. Adjust DiOC(6)3 to a final concentration of 4 nM.
4. Incubate at 37C for 30 mins.
5. Wash cells in PBS.
6. Add DAPI (200 ng/ml)