

Cell Cycle
Analysis Protocol - DAPI Staining
- Harvest
cells washing in PBS.
- Fix
pelleted cells in ice-cold 70% ethanol by adding
with a pasteur pipette
on a vortex. Leave cells at 4oC
from 30 mins to a week.
- Pellet
cells at approximately 2,000 rpm for 5 mins.
- Wash twice in PBS.
- Add
1 mg/ml
of DAPI (Sigma D9542).
- Analyse
by flow cytometry collecting 25,000 events per sample.