


ER Tracker Green labelling Protocol
- Resuspend 0.5 million cells in 1ml of PBS.
- Add
100nM ERTracker Green (MTG) (Invitrogen L7526) for 30 mins at 37C.
- Wash
with PBS.
- Add
200ng/ml
of DAPI (Sigma D9542).
- Set the 530/30nm channel to linear.
- Doublet discriminate via Area & Width parameters .
- Analyse
by flow cytometry collecting 25,000 events per sample.