Viablity Protocols

1. Resuspend cells in 0.5 ml PBS.
2. A) Add 5 mg/ml PI or B) Add 2.5 mg/ml 7-AAD or C) Add >200 ng/ml DAPI or D) Add 50 nM ToPro-3 Add 1ul per 100ul cells of DRAQ7 stock solution.
3. Collect 10,000 events as soon as possible, using the required fluorescence channel, see below.
4. PI – 575 nm, 610 nm or 670 nm channels from Argon laser.
5. 7-AAD 670 nm channel from Argon laser.
6. DAPI 440 nm channel from UV laser or Violet diode.
7. ToPro-3 660 nm channel from Red HeNe (633 nm line).
8. DRAQ7 695 nm or 760 nm channel from Red HeNe (633 nm line). DRAQ7 695 nm or 760 nm channel from Argon laser.