Viablity Protocols

1. Resuspend cells in 0.5 ml PBS.
2. A) Add 5 mg/ml PI or B) Add 2.5 mg/ml 7-AAD or C) Add >200 ng/ml DAPI or D) Add 50 nM ToPro-3.
3. Collect 10,000 events as soon as possible, using the required fluorescence channel, see below.
4. PI – 575 nm, 610 nm or 670 nm channels from Argon laser.
5. 7-AAD 670 nm channel from Argon laser.
6. DAPI 440 nm channel from UV laser or Violet diode.
7. ToPro-3 660 nm channel from Red HeNe (633 nm line).