Automatic Cell Depostion Unit

 

Single cell cloning with the BD FACSAria

The cell sorter at ICMS is fitted with a robotic arm that allows the single cell cloning of required events, this is more proficient than cloning by limting dilutions as the FACAria takes approximately 3 minutes to clone single cells into a 96 well plate. The Aria can also clone into other tissue cluture plates in a sterile manner e.g. 6, 24, 48 and 384 well plates.

The software also allows the operator to put a required number of cells into a well then sort a different population of cells in the next well. In this sort mode only a single population of cells can be sorted at the same time.

The robotic arm also allows sorting onto microscope slides.