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A Cossarizza, G Warnes et al. Guidelines for the use of flow cytometry and cell sorting in immunological studies, Eur J Imm, 2019. (manuscript in preparation).

A Vossenkamper, G Warnes. Flow cytometry reveals the nature of oncotic cells, International J Mol Sci, 2019. (manuscript under review)

A Vossenkamper, G Warnes. Thapsigargin induced apoptosis occurs with a delayed DNA damage response, 2019. (in press Br J Cancer Res).

G Warnes. Determining the fate of cells with flow cytometry, Blog by, Flow Cytometry Congress, London, 2019.

G Warnes. Phenotypic flow cytometric analysis of regulated cell death - 124 sub-populations identified explain why flow cytometry is better here than Western Blot. ACEA Biosciences Webinar, 2019.

J Gibbons, K Marno, R Pike, J Lee, CE Jones, BW Ogunkolade, C Pardieu, G. Warnes, A Prowley, R Sloan, A McKnight. HIV-1 Vpr accessory protein interacts with REAF and mitigates its associated anti-viral activity. PLOS, 2019. (manuscript under review)

H Wan, A Rehman, C Yang, C Hünefeld, H Jedličková, Y Huang, M Teck Teh, J Uttagomol, A Kang, G Warnes, CA Harwood, D Bergamaschi, EK Parkinson, M Röcken, I Hart. The Pemphigus Vulgaris antigen desmoglein-3 suppresses p53 function via the YAP-Hippo pathway. Journal Dermatology, 2019. (manuscript under review)

D Bergamaschi, A Vossenkaemper, WYJ Lee, P Wang, E Bochukova, G Warnes. Simultaneous polychromatic flow cytometric detection of multiple forms of regulated cell death. Apoptosis, 24(5) 453-464, 2019.

A Popat, AA Patel, G Warnes. A flow cytometric study of ER Stress and autophagy, Cytometry A, DOI: 10.1002/cyto.a.23665, 2018.

A Vossenkamper, G Warnes. A flow cytometric immunophenotyping approach to the detection of regulated cell death processes. Journal of Immunological Sciences, 2 (5) 2018.

HL Lee, R Pike, MHA Chong, A Vossenkamper, G Warnes. Simultaneous flow cytometric immunophenotyping of necroptosis, apoptosis and RIP1 dependent apoptosis Methods, 1, 134-135, 56-66, 2018.

S Sisay, L Lopez-Lozano, M Mickunas, A Quiroga-Fernandez, J Palace, G Warnes, R Alvarez-Lafuente, P Dua, U Meir. Untreated relapsing remitting multiple sclerosis patients show antibody production against Latent Epstein Barr Virus (EBV) antigens mainly in the periphery and innate immune IL-8 responses preferentially in the CNS. Journal of Neuroimmunology, 306, 40-45, 2017.

E Pantazi, E Gemenetzidis, MT Teh, SV Reddy, G Warnes, C Evagora, G Trigiante, MP Philpot.
GLI2 is a novel regulator of β-catenin and is associated with loss of E-cadherin, cell invasiveness and long-term epidermal regeneration. Journal of Investigative Dermatology, Mar 11. pii: S0022-202X(17)31218-6. doi: 10.1016/j.jid.2016.11.046. [Epub ahead of print] 2017.

AA Patel, G Warnes. Cell cycle analysis of ER stress and autophagy, Autophagy, Ed N Gorbunov, INTECH pub, ISBN 978-953-51-4815-9, 2016.

ESICM LIVES 2016: part one : Milan, Italy. 1-5 October 2016. DJ Klionsky, G Warnes et alGuidelines for the use and interpretation of assays for monitoring autophagy. Intensive Care Med Exp. Sep;4(Suppl 1):27. doi: 10.1186/s40635-016-0098-x. 2016.

G Warnes. The multiplexing of assays for the measurement of early stages of apoptosis by polychromatic flow cytometry. Flow Cytometry- Recent Prospectives, Ed I Schmid, INTECH pub, ISBN 978-953-51-4346-8, 2016.

G Warnes.Flow cytometric measurement of autophagy: Get yourself to eat your way out of cancer and neurodegenerative diseases. Science Atlas Feb, 2016. 

DJ Klionsky, G Warnes et alGuidelines for the use and interpretation of assays for monitoring autophagy (3rd Edition). Autophagy Jan 2 12(1), 1-222, 2016. 

HDT Torrance, K Brohi, G Warnes, HC Owens, CJ Hinds, DJ Pennington, MJ O'Dwyer. T helper cell polarization following severe polytrauma. Intensive Care Medicine Experimental, 3 (Suppl 1) A848 DOI.10.1186/2197-425X-51-A848,2015.

G Warnes. Flow cytometric assays for the study of autophagy. Methods, doi:10.1016/j.ymeth.2015.03.027, 2015.

A Chikh, P Sanza, C Raimondi, O Akinduro, G Warnes, G Chiorino, C Byrne, CA Harwood, and D Bergamaschi. IASPP is a novel autophagy inhibitor in keratinocytes. Journal of Cell Science Apr28, 2014.

G Warnes. Measurement of autophagy by flow cytometry, Studies of Cell Function. Curr Protoc Cytom. Apr 1;68:9.45.1-9.45.10, 2014.

P Dua, G Warnes, A Chruch, D Martino, D Baker, G Giovannoni, UC Meier.  Functional effects of anti-neuronal antibodies from patients with Tourette's syndrome and disorders associated with streptococcal infection. Neurology Psychiatry and Brain Research; 20(1):17–18 2014.

TS MacFie, R Poulsom, A Parker, G Warnes, TBoitsova, A Nijhuis, N Suraweera, APoehlmann, JSzary, R Feakins, R Jeffery, RW Harper, AM Jubb, JO Lindsay, A Silver.  DUOX2 and DUOXA2 form the predominant enzyme system capable of producing the reactive oxygen species H2O2 in active ulcerative colitis and are modulated by 5-aminosalicylic acid.  Inflammatory Bowel Disease, Jan 2014.

S Chikte, N Panchal, G Warnes. Use of LysoTracker dyes: A flow cytometric study of autophagy. Cytometry A Feb, 85(2), 169-178, 2014.

E Pantazi, E Gemenetzidis, G Trigiante, G Warnes, L Shan, X Mao, M Ikram, MT Teh, YJ Lu, M Philpott.GLI2 induces genomic instability in human keratinocytes by inhibiting apoptosis. Cell Death Dis. Jan 30.5:e1028 doi 10.1038/cddis 2013.535 2014.

E Karanasios, A Englezou, S Cohen, G Warnes. Analysis of Autophagy Regulation: Discussion of recent research and new technologies. Meeting report of the flow cytometry Euroscicon meeting in UK 3rd October 2013. Kindle Edition, Amazon, Honnao Publishing, Jan 2014.

Helgason V. (Meeting Chaired by G Warnes). Autophagy regulation: recent reasearch and new technologies. The Biomedical Scientist 57(12):722-724, 2013.

Luo J, Lee S, Wu D, Yeh J, Ellamushi H, Wheeler AP, Warnes G, Zhang Y, Bo X. P2X7 purinoceptors contribute to the death of Schwann cells transplanted into the spinal cord. Cell Death Dis. Oct 3;4:e829. doi: 10.1038/cddis.2013.343, Oct 2013.

A Want, S Cohen, G Warnes. Cell suicide is not painless for the flow cytometrist: New Assays to measure cell death. Meeting report of the flow cytometry Euroscicon meeting in UK 7th November 2012. Kindle Edition, Amazon, Honnao Publishing, June 2013.

N Panchal, S Chikte, BR Wilbourn, UC Meier, G Warnes. Flow cytometric measurement of cell organelle autophagy. Autophagy - A Double-Edged Sword - Cell Survival or Death? Ed, Y. Bailly. InTech Open Access Publisher ISBN 978-953-51-1062-0, April 2013.

RN Matin, A Chikte, SLP Chong, D Mesher, M Graf, P Sanza, V Senatore, M Scatolini, F Moretti, IM Leigh, CM Proby, A Costanzo, G Chiorino, R Cerio, CA Harwood, D Bergamaschi. p63 is an alternative p53 repressor in melanoma that confers chemoresistance and a poor prognosis. J Exp Med Feb, 210 (3), 581-603, 2013.

C Raimondi, A Chikh, AP Wheeler, T Maffucci, M Falasca. A novel regulatory mechanism links PLCg1 to PDK1. J Cell Sci Epub May18 2012.

KD Henseleit, AP Wheeler, G Warnes, KM Braun. Complementary Whole mount Visualization and Flow Cytometry Strategies. Progenitor Cells : Methods in Molecular Biology Vol 916 (3) p243-261. Eds KA Mace, KM Braun. ISBN 978-1-61779-979-2, 2012.

A Rosello, G Warnes, UC Meier. Cell death pathways and autophagy in the central nervous system and its involvement in neurodegeneration, immunity and CNS infection:  to die or not to die - that is the question. Clinical & Experimental Immunology Apr, 162(1), 52-7, 2012.

DD Ateh, VH Leinster, SR Lambert, A Shah, A Khan, H Walklin, J Johnstone, N Ibrahim, M Kadam, Z Malik, M Girones, G Veldhuis, G Warnes, S Marino, I McNeish, JE Martins. The intracellular uptake of CD95 modified paclitaxel-loaded poly(lactic-co-glycolic acid) microparticles Biomaterials 32 (33), 8538-47, 2011.

SN Jayasinghe, G Warnes, CJ Scotton. Bio-electrosprayed living microenvironments implanted into mouse models. Macromolecular Bioscience 11(10), 1364-69, 2011.

G Warnes, S Martins. Real-time flow cytometry for the kinetic analysis of oncosis. Cytometry 79A, 181-191, 2011.

A. Tarnok. Review of Real-time flow cytometry for the kinetic analysis of oncosis. Cytometry 79A, 177-178, 2011.

R.Challis, G Warnes. Flow cytometry techniques and instrumentation: keeping up with the changes. The Biomedical Scientist 55 (2) : 94-96, 2011.

A Vossenkamper, O Marches, PD Fairclough, G Warnes, AJ Stagg, JO Lindsay, PC Evans,LA Luong, NM Croft, S Naik, G Frankel, TT McDonald. Inhibition of NF-kB signaling in human dendritic cells the enterpathogenic Escherichia coli effector protein NIeE . J Immunol 185: 4118-4127, 2010.

MG Valorani, A Germani, WR Otto, L Harper, A Biddle, CP Khoo, WR Lin, MI Hawa, P Tropel, MP Patrizi, P Pozzilli, MR Alison. Hypoxia increases Sca-1/CD44 co-expression in murine mesenchymal stem cells and enhances their adipogenic differentiation potential. Cell Tissue Res, DOI 10.1007/s00441-010-0982-8 2010.

G Warnes. Flow Cytometric Applications of FRET - Fluorescence Resonance Energy Transfer. The Biomedical Scientist, 53(2) p119-121,Feb 2009.

CP Khoo, MG Valorani, M Brittan, MR Alison, G Warnes, U Johansson, M Hawa, P Pozzilli. Characterization of endothelial progenitor cells in the NOD mouse as a source for cell therapies. Diabetes Metab Res Rev 25(1): 89-93, 2009.

SM Janes, TA Ofstad, DH Campbell, A Eddaoudi, G Warnes, D Davies, F Watt. PI3-kinase dependent activation of apoptotic machinery occurs on commitment of epidermal keratinocytes to terminal differentiation. Cell Res 19(3): 328-339, 2009.

JP Biggerstaff, B Weidow, J Dexheimer, G Warnes, J Vidosh, S Patel, M Newman, P Patel. Soluble fibrin inhibits lymphocyte adherence and cytotoxicity against tumor cells:Implications for cancer metastasis and immunotherapy. Clin Appl Thromb Hemost 14(2): 193-202, 2008.

P Takousis, P Johonnett, J Williamson, P Sasieni, G Warnes, T Forshew, V Azuara, A Fisher, PJ Wu, PJ Jones, R Vatcheva, S Beck, D Sheer. Replication timing profile reflects the distinct functional and genomic features of the MHC Class II Region. Cell Cycle Oct 6:19, 2393-2398, 2007.

S Leverrier, D Bergamaschi, L Ghali, A Ola, G Warnes, B Akgul, K Blight, R Garcia-Escudero, A Penna, A Eddaoudi, A Storey. Role of HPV E6 proteins in preventing UVB-induced release of pro-apoptotic factors from the mitochondria. Apoptosis 12 (3), 549-60, 2007.

G Warnes, MG Macey. Calcium: Cytoplasmic, mitochondria, endo-plasmic reticulum and flux measurements, Flow Cytometry: Principles & Applications. Ed MG Macey, Springer, Humana Press ISBN 978-1-58829-691-7, 2007.

V Azuara, P Perry, S Sauer, M Spivakov, HF Jorgensen, RM John, M Gouti, M Casanova, G Warnes, M Merkenschlager, AG Fisher. Chromatin signatures of pluripotent cell lines. Nature Cell Biol. 8, 532-538, 2006.

P Perry, S Saur, N Billion, WD Richardson, M Spivakov, G Warnes, FJ Livesey, M Merkenschlager, AG Fisher, V Auara. A dynamic switch in the replication timing of key regulator genes in embryonic stem cells upon neural induction. Cell Cycle 3, (12), 1645-50, 2004.

MR Ehrenstein, JG Evans, A Singh, S Moore, G Warnes, DA Isenberg, C Mauri. Compromised function of regulatory T cells in Rheumatoid Arthritis and reversal by anti-TNF therapy. J Exp Med. 200, 277-285, 2004.

P Sabbattini, M Lundgren, A Georgiou, C Chow, G Warnes, N Dillion. Binding of Ikaros to the lambda5 promoter silences transcription through a mechanism that does not require heterochromatin formation. EMBO J. 20(11), 2812-22, 2001.

PH Dixon, et al, G Warnes, C Williamson. Heterozygous MDR3 missense mutation associated with intrahepatic cholestasis of proegnancy: evidence for a defect in protein trafficking. Hum Mol Genet. 9(8), 1209-17, 2000.

G Warnes, JP Biggerstaff, JL Francis. Monocyte B7 and Sialyl Lewis X modulates the efficacy of IL-10 down-regulation of LPS-induced monocyte tissue factor in whole blood. British Journal of Haematology 102,588-596, 1998.

VA Gant, G Warnes, I Phillips, GF Savidge. The application of flow cytometry to the study of bacterial responses to antibiotics. J Med Microbiol 39,1475-154, 1993.


by Gary Warnes. © Queen Mary, University of London 2007
Institute of Cell and Molecular Science, The Blizard Building, Barts and The London School of Medicine and Dentistry, 4 Newark Street, London E1 2AT, UK Tel: +44 (0)20 7882 2483, Fax: +44 (0)20 7882 2200