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  Institute of Cell and Molecular Science
Flow Cytometry Core Facility equipment, images used with permission by BD
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Replication Timing Studies

 

Fractional S-phase sorting using bromodeoxyuridine uptake to study gene replication timing

The determination of the percentage of cells in S-phase is dependent upon the detection of a thymidine analogue, bromodeoxyuridine (BrdU), which when added to culture medium is incorporated into DNA during DNA replication is a well known technique in flow cytometry.

Gene replication timing studies uses the BrdU labelling technique, S phase cells are sorted into 3 or more fractions. Collected fractions are analysed by PCR for genetic analysis after immunoprecipitation of DNA via incorporated BrdU.

 
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PI S Phase Sorting

 

BrdU S Phase Sorting
[click for large version]
by Gary Warnes. © Queen Mary, University of London 2007
Institute of Cell and Molecular Science, Barts and The London, Queen Mary's School of Medicine and Dentistry, Queen Mary, University of London, 4 Newark Street, London E1 2AA, Tel: +44 (0)20 7377 7000, Fax: +44 (0)20 7247 3428