For the identification of small populations of cells (<5%) it is important to ensure that sufficient events are recorded to provide precision. A simple calculation, r = (100/CV) 2 where r is the number of events meeting the required criterion, can be used to   determine that size of the database that will provide a given precision.

This calculation has been used to construct a table [PDF 44KB] which can be used as a guide in all cases of rare event analysis.

Publications.

KD Henseleit, AP Wheeler, G Warnes, KM Braun. Complementary Whole mount Visualization and Flow Cytometry Strategies. Progenitor Cells : Methods & Protocols. Methods in Molecular Biology Vol 916. Eds KA Mace, KM Braun. ISBN 978-1-61779-979-2, 2012.

MG Valorani, A Germani, WR Otto, L Harper, A Biddle, CP Khoo, WR Lin, MI Hawa, P Tropel, MP Patrizi, P Pozzilli, MR Alison. Hypoxia increases Sca-1/CD44 co-expression in murine mesenchymal stem cells and enhances their adipogenic differentiation potential. Cell Tissue Res, DOI 10.1007/s00441-010-0982-8 2010.

CP Khoo, MG Valorani, M Brittan, MR Alison, G Warnes, U Johansson, M Hawa, P Pozzilli. Characterization of endothelial progenitor cells in the NOD mouse as a source for cell therapies. Diabetes Metab Res Rev 25(1): 89-93, 2009.