DNA content may be measured in association with other nuclear or cellular components such as cyclins by dual fluorescence analysis in which PI/DAPI is used to stain the DNA and for example FITC or APC conjugated antibody is used to define the antigen of interest. When using DAPI most antibody fluorophores can be used except for Pacific Blue. Whilst with PI which emits at 619nm, PE, PerCP or PE-Cy5 or conjugated antibodies cannot be used as as PI emits at the same wavelength as these fluorophores.

Surface immunophenotyping can also be used in conjunction with DNA content using PI or DAPI, see protocols below.

• 3-D histogram plot
• PI cell cycle analysis with intracellular antigen protocol
• 7-AAD cell cycle analysis and intracellular antigen staining
• DAPI cell cycle analysis with intracellular antigen staining protocol
• PI cell cycle analysis with surface staining protocol
• DAPI cell cycle analysis with surface staining protocol