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  Institute of Cell and Molecular Science
Flow Cytometry Core Facility equipment, images used with permission by BD
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Muse detection of Fluorescent Proteins

 

The Muse instrument can be used to measure fluorescence not from a Millipore kit. Fluorescent protein fluorescence from mCherry can be quantitated by use of the 575nm or PI channel as the Green 532nm laser line excites mCherry and collects the emissionwhich can be displayed if the User collects the fluoresence as a histogram of mCherry fluorescence with an MFI read out using the Cy3 Smartflare program, see figure.

 
 
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mCherry Fluorescence

 

 
by Gary Warnes. © Queen Mary, University of London 2007
Institute of Cell and Molecular Science, The Blizard Building, Barts and The London School of Medicine and Dentistry, 4 Newark Street, London E1 2AT, UK Tel: +44 (0)20 7882 2483, Fax: +44 (0)20 7882 2200