Image - Barts and The London logo and link to home page Image - divider Image - divider
  Institute of Cell and Molecular Science
Flow Cytometry Core Facility equipment, images used with permission by BD
  Flow Cytometry Core Facility
  link Home link Instruments available link Flow cytometry link Uses of flow cytometry link Flow sorting link Links link Contacts

Functional analysis


It is possible to assess changes in cells over short time periods by flow cytometry. These include a wide array of biological activities, from studies such as changes in calcium content in cells in response to antagonists. Mitochondrial membrane potential changes and Reactive Oxygen Species ROS and nitric oxide generation during apoptosis. Mitochondrial Transition Pore (MTP) opening can also be monitored during apoptosis (see other assays).

Cell proliferation can also be measured by the use of CFSE or Cell Tracker Qdots. The rate of phagocytosis can be determined by the use of fluorescently labelled bacteria. (see cell proliferation section).

FRET can be used to detect the proximity of cell molecules via the use of fluorescently labelled monoclonal antibodies or Fluorescent Protein (FP) molecules (see FRET section).


PH Dixon, et al, G Warnes, C Williamson. Heterozygous MDR3 missense mutation associated with intrahepatic cholestasis of pregnancy: evidence for a defect in protein trafficking. Hum Mol Genet. 9(8), 1209-17, 2000.

by Gary Warnes. © Queen Mary, University of London 2007
Institute of Cell and Molecular Science, The Blizard Building, Barts and The London School of Medicine and Dentistry, 4 Newark Street, London E1 2AT, UK Tel: +44 (0)20 7882 2483, Fax: +44 (0)20 7882 2200