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Antibody Binding Kinetic Studies

 

The binding of fluorescently labelled monoclonal antibodies (mcab) to cell plasma membranes can be studies in real-time. Cells of interest are initially acquired for 30 seconds to generate a baseline control of Fluorescence against Time parameters. Antibody is then added to the cells, this is usually followed by a rapid rise in mcab related fluorescence against time. This can be compared to isotype control antibody added to the cells in the same manner.

Below are given examples of anti-CD38-PE binding kinetics, a dose dependent binding kinetic study of anti-CXCR4-APC and a comparison of of the binding kinetics of different clones of anti-CD4-APC.

 
 
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Anti-CD38-PE binding

ant-CXCR4 dose response kinetics

CD4 clone binding kinetics

by Gary Warnes. © Queen Mary, University of London 2007
Institute of Cell and Molecular Science, Barts and The London, Queen Mary's School of Medicine and Dentistry, Queen Mary, University of London, 4 Newark Street, London E1 2AA, Tel: +44 (0)20 7377 7000, Fax: +44 (0)20 7247 3428