HomeRequirements
Potential users are requested to complete a "Flow Cytometry User Form" see below. This contains questions relating to the proposed flow cytometry use and will enable the user to determine whether the proposed use is feasible, how long the usage will be and so cost.
Sample preparation
For multicolour analysis there is a requirement for single labelled cells or FACSComp beads to be prepared. This will then allow automatic compensation to be performed on the instruments.
All samples for sorting must be filtered (70μm). Tissue culture medium containing serum and antibiotics should be provided for sort collection.
Costing
The cost of usage will be based on the training required, consultation time, set up time, number of samples analysed and the number and duration of sorts. ICMS Staff and associated Institutes costs will be £25 per hour for analysis, £40 per hour for sorting. External Academic costs are £40 per hour for analysis, £60 per hour for sorting. Industrial costs are £60 per hour for analysis and £100 per hour for sorting.
Data analysis
For post acquisition data analysis FlowJo is available. Personal copies may be obtained from http://flowjo.com. [new window]
Training
It is important that all users of the flow cytometers are trained to use the instruments to a minimum standard. In house training will be available.