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Platelet Analysis and Cell Counting


Human and murine platelets from whole blood can be immunophenotyped by the labelling of GpIb and GpIIb/IIIa to determine the presence of the respective clinical syndromes Glanzmann's Thrombasthenia and Bernard-Soulier syndrome and also the counting of platelet numbers.

The homozygote double recessive and heterozygote conditions of Glanzmann's Thrombasthenia has a >90% and approximately 50% reduced expression of GpIIb/IIIa respectively. Bernard-Soulier's syndrome showing a marked reduction in GpIb expression that can be readily shown by flow cytometric analysis after immunophenotyping of GpIb.

Protocols :

by Gary Warnes. © Queen Mary, University of London 2007
Institute of Cell and Molecular Science, The Blizard Building, Barts and The London School of Medicine and Dentistry, 4 Newark Street, London E1 2AT, UK Tel: +44 (0)20 7882 2483, Fax: +44 (0)20 7882 2200