There are a wide variety of commercially available fluorochromes conjugated and unconjugated to monoclonal antibody reagents which are used to detect a wide variety of surface antigens. The choice of fluorochromes is limited by the laser excitation sources available in your flow cytometer(s). The instruments available at the ICMS core facility allow a most of the commercially available fluorochromes to be used with some limitations. The tables below show the fluorochromes and cell dyes and stains that can be analysed by flow cytometry at the BICMS flow cytometry core facility.
- Antibody fluorochromes- UV
- Antibody fluorochromes- Violet
- Antibody fluorochromes- Blue
- Antibody fluorochromes- Yellow-Green
- Antibody fluorochromes- Red
- Relative Fluorochrome Brightness
- BB515
- DNA stains
- Organelle stains
- Reactive Oxygen Species stains
- Calcium Dyes
- Miscellaneous stains
- Fluorescent Proteins
- Fluorochrome Spectral Viewer
Short list of fluorochromes, cell dyes & stains available
- Antibody fluorochromes, cell dyes & stains [PDF - 52KB]
Quantum dots can also be used on the flow cytometers at ICMS, these include Qdot 525, Qdot 585, Qdot 605, Qdot 655, for details about the use of Qdots please go to the section on Quantum Dots.