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  Institute of Cell and Molecular Science
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Fluorochromes at The Blizard

 

There are a wide variety of commercially available fluorochromes conjugated and unconjugated to monoclonal antibody reagents which are used to detect a wide variety of surface antigens. The choice of fluorochromes is limited by the laser excitation sources available in your flow cytometer(s). The instruments available at the ICMS core facility allow a most of the commercially available fluorochromes to be used with some limitations. The tables below show the fluorochromes and cell dyes and stains that can be analysed by flow cytometry at the BICMS flow cytometry core facility.

Short list of fluorochromes, cell dyes & stains available

Quantum dots can also be used on the flow cytometers at ICMS, these include Qdot 525, Qdot 585, Qdot 605, Qdot 655, for details about the use of Qdots please go to the section on Quantum Dots.

 
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by Gary Warnes. © Queen Mary, University of London 2007
Institute of Cell and Molecular Science, The Blizard Building, Barts and The London School of Medicine and Dentistry, 4 Newark Street, London E1 2AT, UK Tel: +44 (0)20 7882 2483, Fax: +44 (0)20 7882 2200