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Muse Smartflare Kit

The Millipore Smartflare kits use Cy3 and Cy5 as fluorescent tags and thus Cy3 can be analyzed on a Muse for those Users without access to a Yellow-Green (561nm) laser. There are over 50 different mRNA biologically specific kits available. The technology employs a gold nanoparticle taht when unbound to it's target quenches the Cy3 (or Cy5) conjugated to the gold particle, see figure below.

Excitation & Emission of Smartflares

Muse Kit Results

 

 
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by Gary Warnes. © Queen Mary, University of London 2007
Institute of Cell and Molecular Science, The Blizard Building, Barts and The London School of Medicine and Dentistry, 4 Newark Street, London E1 2AT, UK Tel: +44 (0)20 7882 2483, Fax: +44 (0)20 7882 2200