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  Institute of Cell and Molecular Science
Flow Cytometry Core Facility equipment, images used with permission by BD
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Bone Marrow

 

Four colour labelling

Murine bone marrow was analysed for endothelial cell stem cells by labelling with negative selection cocktail-FITC (CD45, B220, etc), CD31-PE, Sca-1-PE-Cy7 and c-kit-APC and back-gating onto FSC vs SSC after doublet discrimination, see figures for identification of endothelial stem cells and control panel.

 

Commonly used monoclonal antibody fluorophores are listed in fluorochromes used at ICMS table.

 
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4 colour Immunophenotyping

 

by Gary Warnes. © Queen Mary, University of London 2007
Institute of Cell and Molecular Science, The Blizard Building, Barts and The London School of Medicine and Dentistry, 4 Newark Street, London E1 2AT, UK Tel: +44 (0)20 7882 2483, Fax: +44 (0)20 7882 2200