Murine bone marrow was analysed for endothelial cell stem cells by labelling with negative selection cocktail-FITC (CD45, B220, etc), CD31-PE, Sca-1-PE-Cy7 and c-kit-APC and back-gating onto FSC vs SSC after doublet discrimination, see figures for identification of endothelial stem cells and control panel.
Commonly used monoclonal antibody fluorophores are listed in fluorochromes used at ICMS table.