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FRET based gene reporter systems


FRET based gene reporter systems

A gene reporter system developed by Invitrogen uses FRET-based fluorescent substrates, CCF2-AM and CCF4-AM. These substrates are loaded easily into cells via the AM ester and produce a green FRET signal (520 nm) when excite with a Violet diode. Beta-lactamases produced as a by-product of gene expression cleaves the FRET-based substrate resulting in a change of emission to 447 nm, see figure.






FRET based gene reporting system

CCF2 mode of action

Flow cytometric analysis with CCF2

FRET Efficiency Ratio Formula


by Gary Warnes. © Queen Mary, University of London 2007
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