| Specification |
Comments |
| Performance |
| Fluorescence sensitivity |
<200 molecules of equivalent soluble fluoroscein |
| Fluorescence resolution |
< 3% for PI labelled chick erythrocyte nuclei |
| Forward and side scatter sensitivity |
Sensitivity enables the separation of fixed platelets from noise |
| Forward and side scatter resolution |
>10,000 events/sec |
| Excitation optics |
| Optical platform |
Fixed optical assembly, kinematic beam steering |
| Lasers |
20mW Coherent Sapphire 488nm, air cooled, argon ion laser: 530/30nm; 585/42nm; 670nmLP; 780/60nm.
25 mW 405 Coherent VioFlame; 450/50nm; 510/50nm
20 mW 635 JDS Uniphase HeNe; 660/20nm; 780/60nm. |
| Emission optics |
| Optical Coupling |
Quartz cuvette is coupled to emission lens by refractive index-matching optical gel for optimum collection efficiency |
| Fluorescence Detectors |
Three fixed fibre apertures |
| Eight fluorescence detectors |
High performance, high dynamic range photomultipliers with filters |
| Signal processing |
| Workstation resolution |
Five decades for peak detection, offset less than 1/10,000 full scale |
| Data acquisition channels |
Ten acquisition channels with up to three time-displaced laser beams |
| Dynamic range |
18-bit data acquisition with IEEE 32 bit floating-point resolution
262,144 linear channels displayed in a 5-decade logarithmic display |
| Pulse processing |
Width and area measurements available for all fluorescence parameters
Ratio measurements for intra-laser parameters |
| Time |
Time available correlated to any parameter |
| Channel triggering |
Available on any laser signal |
| Data management |
| Central processing |
Dual-Core Intel Xeon processors |
| Data storage |
2GB RAM and 120-GB internal hard drive, internal drive with 250-MG removable disk storage space capacity, 3.5-inch floppy drive, CD-RW drive. |
| Monitor |
24-inch LCD monitor |
| Software |
BD FACSDiva v6.1.3, BD FACSCanto clinical v6.1.03 |
| Data File Structure |
Flow Cytometry Standard 3.0 or 2.0
|
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