Specification | Comments |
Performance | |
Fluorescence sensitivity | FITC 125 molecules of equivalent soluble fluorochrome
PE 125 molecules of equivalent soluble fluorochrome |
Fluorescence resolution | CV < 3% for PI labelled chick erythrocyte nuclei CV < 3% for Hoechst labelled chick erythrocyte nuclei |
Forward and side scatter sensitivity | Sensitivity enables the separation of fixed platelets from noise
Identification of bacteria and 0.5 micron beads |
Forward and side scatter resolution | 20,000 events/sec |
Excitation optics | |
Optical platform | Fixed optical assembly, upon cuvette flow cell |
Lasers | 20mW Coherent Sapphire blue 488nm: 530/30nm: 695/40nm |
Emission optics | |
Optical Coupling | Quartz cuvette is coupled to emission lens by refractive index-matching optical gel for optimum collection efficiency |
Fluorescence Detectors | Three fixed fibre apertures |
Twelve fluorescence detectors | High performance, high dynamic range photomultipliers with filters |
Steering Optics | Fibre optics steer the three laser beams onto the beam expansion prisms, and then are focused upon the cuvette flow cell |
Signal processing | |
Workstation resolution | Five decades for peak detection, offset less than 1/10,000 full scale |
Data acquisition channels | 17 parameters, 15 fluorescent and two scatter |
Dynamic range | 18-bit data acquisition 262,144 linear channels displayed in a 5-decade logarithmic display |
Pulse processing | Width and area measurements available for all fluorescence parameters Ratio measurements for intra-laser parameters |
Time | Time available correlated to any parameter |
Channel triggering | Available on any laser signal |
Data management | |
Central processing | Dual Intel Core 2 CPU |
Data storage | 2GB RAM, 300-GB internal hard drive, 3.5-inch floppy drive, CD-RW drive |
Data File Structure | Flow Cytometry Standard 3.0 or 2.0 |
Software | BD FACSDiva software v6.1.03 |
Fluidics | |
General | Fluidics cart provides sheath and cleaning fluid to instrument and receives waste
No air or vacuum required Adjustable sheath pressure |
Sample flow rate | Adjustable |
Sample injection chamber | |
Sample input agitation | Adjustable through software |
Temperature control | Software adjustable, 4, 20, 37 and 42 o C |
Aerosol management | |
Standard feature Aerosol Management System - AMS- fitted with Buffalo HEPA filter |
Sample injection chamber and sample collection tube area provide a sealed area to minimize aerosols. |
Sort performance | |
Sort collection devices | Two way sorting and four way sorting in to sample tubes 70um, 85um and 100um diameter nozzle sizes available for sorting at 20,000, 10,000 and 5,000 cells per second at 70, 20 and 12 psi. |