Specification | Comments |
Performance | |
Fluorescence sensitivity | <35 molecules of equivalent soluble fluoroscein |
Fluorescence resolution | < 3% for PI labelled chick erythrocyte nuclei |
Forward and side scatter sensitivity | Sensitivity to detect 200 nm particles |
Forward and side scatter resolution | 35,000 events/sec |
Excitation optics | |
Optical platform | Fixed optical assembly, |
Lasers | Blue, Yellow-Green, Violet, Red & UV lasers with 96 well plate reader |
Emission optics | |
Optical Coupling | Quartz cuvette is coupled to emission lens by refractive index-matching optical gel for optimum collection efficiency |
Fluorescence Detectors | 64 high performance PMTs, Blue laser 14 parameters, Violet 16, Red 8, UV 16 and Yellow-Green laser 10 parameters |
40 fluorescence detectors | High performance, high dynamic range photomultipliers with spectral unmixing |
Signal processing | |
Workstation resolution | 6.5 decades for area detection with spectral unmixing |
Data acquisition channels | 40 acquisition channels |
Dynamic range | 22-bit data acquisition 6.5-decade logarithmic display with pre-set voltages |
Pulse processing | available |
Time | Time available correlated to any parameter |
Digital spectral unmixing | Electronics provides spectral unmixing |
Data management | |
Central processing | Intel processor |
Spectroflow Software |
Windows 10 computer |
Monitor | 20-inch LCD monitors |
Data File Structure | Flow Cytometry Standard 3.1 |