Image - Barts and The London logo and link to home page Image - divider Image - divider
 
  Institute of Cell and Molecular Science
Flow Cytometry Core Facility equipment, images used with permission by BD
  Flow Cytometry Core Facility
  link Home link Instruments available link Flow cytometry link Uses of flow cytometry link Flow sorting link Links link Contacts
 
Cytek Aurora Spectral Analyser
Specification Comments
Performance
Fluorescence sensitivity <35 molecules of equivalent soluble fluoroscein
Fluorescence resolution < 3% for PI labelled chick erythrocyte nuclei
Forward and side scatter sensitivity Sensitivity to detect 200 nm particles
Forward and side scatter resolution 35,000 events/sec
Excitation optics
Optical platform Fixed optical assembly,
Lasers Blue, Yellow-Green, Violet, Red & UV lasers with 96 well plate reader
Emission optics
Optical Coupling Quartz cuvette is coupled to emission lens by refractive index-matching optical gel for optimum collection efficiency
Fluorescence Detectors 64 high performance PMTs, Blue laser 14 parameters, Violet 16, Red 8, UV 16 and Yellow-Green laser 10 parameters
40 fluorescence detectors High performance, high dynamic range photomultipliers with spectral unmixing
Signal processing
Workstation resolution 6.5 decades for area detection with spectral unmixing
Data acquisition channels 40 acquisition channels
Dynamic range 22-bit data acquisition
6.5-decade logarithmic display with pre-set voltages
Pulse processing available

Time Time available correlated to any parameter
Digital spectral unmixing Electronics provides spectral unmixing
Data management
Central processing Intel processor

Spectroflow Software

Windows 10 computer
Monitor 20-inch LCD monitors
Data File Structure Flow Cytometry Standard 3.1
 
Top
 
by Gary Warnes. © Queen Mary, University of London 2007
Institute of Cell and Molecular Science, The Blizard Building, Barts and The London School of Medicine and Dentistry, 4 Newark Street, London E1 2AT, UK Tel: +44 (0)20 7882 2483, Fax: +44 (0)20 7882 2200