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| ACEA Novocyte |
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| Specification |
Comments |
| Performance - see pdf |
| Fluorescence sensitivity |
<75 molecules of equivalent soluble fluoroscein |
| Fluorescence resolution |
< 3% for PI labelled chick erythrocyte nuclei |
| Forward and side scatter sensitivity |
Sensitivity 0.2 um |
| Forward and side scatter resolution |
>35,000 events/sec |
| Excitation optics |
| Optical platform |
Fixed optical assembly, kinematic beam steering |
| Lasers |
20mW Blue 488nm, 530/30nm; 585/40nm; 615/24nm; 675/30nm; 780/60nm. (5)
25 mW Violet 405nm 445/45nm; 530/30nm; 585/40nm; 615/24nm; 675/30nm; 780/60nm. (6)
20 mW 640 Red HeNe; 675/30nm; 780/60nm. (2) |
| Emission optics |
| Optical Coupling |
Fixed |
| Fluorescence Detectors |
7 PMTs plus FSC Diode |
| Seven fluorescence detectors |
High performance, high dynamic range photomultipliers with filters |
| Signal processing |
| Workstation resolution |
Seven decades for peak detection |
| Data acquisition channels |
Fifteen acquisition channels with up to three time-displaced laser beams |
| Dynamic range |
24 bit 7.2-decade logarithmic display |
| Pulse processing |
Height and area measurements available for all fluorescence parameters
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| Time |
Time available correlated to any parameter |
| Channel triggering |
Available on any laser signal |
| Data management |
| Central processing |
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| Data storage |
1 TB |
| Monitor |
19-inch LCD monitor |
| Software |
ACEA NovoExpress |
| Data File Structure |
Flow Cytometry Standard 3.0 |
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