Image - Barts and The London logo and link to home page Image - divider Image - divider
 
  Institute of Cell and Molecular Science
Flow Cytometry Core Facility equipment, images used with permission by BD
  Flow Cytometry Core Facility
  link Home link Instruments available link Flow cytometry link Uses of flow cytometry link Flow sorting link Links link Contacts
 
Merck Millipore Muse Digital Analyser
Specification Comments
Performance
Fluorescence sensitivity <2,000 molecules of equivalent soluble
Fluorescence resolution < 3% for PI labelled chick erythrocyte nuclei
Forward scatter sensitivity photodiode
Forward scatter resolution 10,000 to 500,000 particles/ml
sample flow rate 7-36 ul/min
Excitation optics
Optical platform Fixed optical assembly
Lasers 532nm Green HeNe laser
Emission optics
Optical Coupling Guava type flow cell
Fluorescence Detectors 2 Photodiodes
Two fluorescence detectors High performance, high dynamic range photodiodes with filters for PE-PI (576/28nm) and PE-Cy5-7-AAD (680/30nm)
Signal processing
Workstation resolution Four decades for area parameter detection
Data acquisition channels Three acquisition channels
Dynamic range 32-bit data acquisition
Pulse processing Digital

Time Not available
Digital Compensation Electronics provides digital correction of spectral overlap
Data management
Central processing Intel processor

Muse Software

Embedded intel ATOM-based PC with 160GB Hard Drive
Monitor 4-6 inch built in touch screen
Data File Structure Flow Cytometry Standard 3.0
 
Top
 
by Gary Warnes. © Queen Mary, University of London 2007
Institute of Cell and Molecular Science, The Blizard Building, Barts and The London School of Medicine and Dentistry, 4 Newark Street, London E1 2AT, UK Tel: +44 (0)20 7882 2483, Fax: +44 (0)20 7882 2200