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| BD LSR II anaylser |
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| Specification |
Comments |
| Performance |
| Fluorescence sensitivity |
<200 molecules of equivalent soluble fluoroscein |
| Fluorescence resolution |
< 3% for PI labelled chick erythrocyte nuclei |
| Forward and side scatter sensitivity |
Sensitivity enables the separation of fixed platelets from noise |
| Forward and side scatter resolution |
>10,000 events/sec |
| Excitation optics |
| Optical platform |
Fixed optical assembly, kinematic beam steering |
| Lasers |
20mW Coherent Sapphire Blue 488nm, 530/30nm; 575/26nm; 610/20nm; 675/20nm; 695/40nm; 780/60nm.
25 mW 405 Coherent VioFlame 440/40nm; 525/50nm; 610/20nm.
20 mW 635 JDS Uniphase HeNe; 660/20nm; 780/60nm or 695/40nm.
355 nm Lightwave Solid-StateUV laser; 450/40nm; 530/30nm. |
| Emission optics |
| Optical Coupling |
Quartz cuvette is coupled to emission lens by refractive index-matching optical gel for optimum collection efficiency |
| Fluorescence Detectors |
Four fixed fibre apertures |
| Thirteen fluorescence detectors |
High performance, high dynamic range photomultipliers with filters |
| Signal processing |
| Workstation resolution |
Five decades for peak detection, offset less than 1/10,000 full scale |
| Data acquisition channels |
Fifteen acquisition channels with up to four time-displaced laser beams |
| Dynamic range |
18-bit data acquisition
262,144 linear channels displayed in a 5-decade logarithmic display |
| Pulse processing |
Width and area measurements available for all fluorescence parameters
Ratio measurements for intra-laser parameters |
| Time |
Time available correlated to any parameter |
| Channel triggering |
Available on any laser signal |
| Data management |
| Central processing |
Dual Intel Core 2 CPU |
| Data storage |
2GB RAM, 300-GB internal hard drive, 3.5-inch floppy drive, CD-RW drive |
| Monitor |
Two 18-inch LCD monitors |
| Software |
FACSDiva v6.1.03 |
| Data File Structure |
Flow Cytometry Standard 3.0 or 2.0 |
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