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| Becton Dickinson FACScan Analyser |
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| Specification |
Comments |
| Performance |
| Fluorescence sensitivity |
<2,000 molecules of equivalent soluble fluoroscein |
| Fluorescence resolution |
< 3% for PI labelled chick erythrocyte nuclei |
| Forward and side scatter sensitivity |
Sensitivity enables the separation of fixed platelets from noise |
| Forward and side scatter resolution |
5,000 events/sec |
| Excitation optics |
| Optical platform |
Fixed optical assembly, |
| Lasers |
20mW Coherent 488nm, air cooled, argon ion laser
FITC (530/30nm); PE (575/26nm), PerCP, or PE-Cy5,or PE-Cy5-5 or PE-Cy7 (650nmLP) |
| Emission optics |
| Optical Coupling |
Quartz cuvette is coupled to emission lens by refractive index-matching optical gel for optimum collection efficiency |
| Fluorescence Detectors |
3 PMTs |
| Three fluorescence detectors |
High performance, high dynamic range photomultipliers with filters for FITC, PE and PE-Cy5 or PE-Cy7 |
| Signal processing |
| Workstation resolution |
Four decades for peak detection, |
| Data acquisition channels |
Five acquisition channels |
| Dynamic range |
12-bit data acquisition
1,024linear channels displayed in a 4-decade logarithmic display |
| Pulse processing |
Not available
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| Time |
Time available correlated to any parameter |
| Analogue Compensation |
Electronics provides analogue correction of spectral overlap |
| Data management |
| Central processing |
Intel processor |
CellQuest Pro II
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Apple Mac G5 computer with OSX operating system |
| Monitor |
15-inch LCD monitors |
| Data File Structure |
Flow Cytometry Standard 2.0 |
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